This video is part of a series illustrating proper laboratory spreading/streaking technique. This video demonstrates flaming an innoculating loop using a bunsen burner and aseptic technique, then cooling the loop in agar and streaking a plate with the loop. This technique is taught in Introduction to Cell Biology: Biology 107 and Molecular Genetics and Heredity: Biology 207. This video was created as part of the University of Alberta OER database project in Biological Sciences. Identifier 3003V.